Pro-angiogenic and osteogenic composite scaffolds of fibrin, alginate and calcium phosphate for bone tissue engineering

Due to the limitations of bone autografts, we aimed develop new composite biomaterials with pro-angiogenic and osteogenic properties be used as scaffolds in tissue engineering applications. We a porous, cross-linked slowly biodegradable fibrin/alginate scaffold originally developed our laboratory for wound healing, throughout which deposits calcium phosphate (CaP) were evenly incorporated using an established biomimetic method. Material characterisation revealed porous nature confirmed deposition CaP precursor phases scaffolds. MC3T3-E1 cells adhered scaffolds, proliferated, migrated differentiated down pathway during culture period. Chick chorioallantoic membrane (CAM) assay results showed that biocompatible. The work presented here gave useful insights into potential these merits further research pre-clinical model prior its clinical translation.